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Alinity hq

NORMAL CASE

Description

This case represents an adult, displaying what is considered a normal hematological profile, with all results within the reference ranges. The user interface screen is shown below, as well as an enlarged image and description of each scatterplot and histogram in the following figures.

Numerical Results

 
WBC 5.89 10e3/μL
NEU 3.73 10e3/μL 63.3 %
LYM 1.49 10e3/μL 25.3 %
MONO 0.42 10e3/μL 7.12 %
EOS .235 10e3/μL 3.99 %
BASO .015 10e3/μL .261 %
IG 0.00 10e3/μL .007 %
NRBC 0.00 10e3/μL
NR/W 0.00

 
RBC 5.35 10e6/μL
HGB 14.1 g/dL
HCT 47.3 %
MCV 88.6 fL
MCH 26.3 pg
MCHC 29.7 g/dL
RDW 12.5 %

 
RETIC 78.7 10e3/μL 1.47 %
IRF .315
MCHr 25.1 pg

 
PLT 266. 10e3/μL
MPV 7.25 fL
%rP 3.78 %

 
FIGURE 1. IAS x ALL (WBC):
Intermediate angle vs. size
Neutrophils and immature granulocytes (yellow), and eosinophils are separated from lymphocytes (cyan) and monocytes (purple) on the basis of their size, IAS, and 90° polarized side scatter (PSS) light scatter characteristics (representing size, complexity and lobularity, respectively).
FIGURE 2. ALL x PSS (WBC):
Size vs. polarized side scatter
In this scatterplot, nuclear lobularity (PSS) is plotted as a function of cell size (ALL). This plot is especially useful in the detection of platelet clumps and for demonstrating the degree of nuclear segmentation of neutrophils.
FIGURE 3. FL1 x ALL (WBC):
Fluorescence vs. size
Fluorescence (x-axis) vs. size (y-axis) provides separation of low and high fluorescence cells, which facilitates enumeration of nucleated red blood cells (NRBC).
FIGURE 4. PSS x DSS (WBC):
Polarized side scatter vs. depolarized side scatter
Eosinophils (green) are differentiated from neutrophils (yellow) by their unique ability to depolarize 90° light (90° DSS).
FIGURE 5. CHC x Volume (RBC):
RBC cellular hemoglobin content vs. RBC volume
RBC cellular hemoglobin concentration (x-axis) vs. RBC size (y-axis) is depicted here. The entire population of RBC is shown, and deviation from normal in terms of microcytosis, macrocytosis, hypochromia and hyperchromia is visualized by four fixed thresholds that define hypochromic (< 28 g/dL), hyperchromic (> 41 g/dL), microcytic (< 60 fL) and macrocytic (> 120 fL) RBC.
FIGURE 6. Volume (PLT):
Platelet volume
Platelet size (x-axis) vs. number (y-axis) are shown here. This plot is especially useful for demonstrating increased numbers of large/giant platelets and very small RBC. The histogram shows platelet volume distribution, which is usually log normal.
FIGURE 7. IAS2 X ALL (PLT):
Intermediate angle light scatter vs. size
Platelets (yellow) are identified based on their cellular complexity (IAS2) and size (ALL). This plot is especially useful for separating platelets from noise and other interfering substances.
FIGURE 8. Volume (RBC):
RBC volume histogram
RBC normally exhibit a Gaussian distribution of their volume. The mean corpuscular volume (MCV) can be seen where the curve peaks. The RBC distribution width (RDW) is calculated from standard deviation (SD) and the mean (MCV), and shows RBC size and the degree of anisocytosis (RDW).
FIGURE 9. ALL x IAS3 (RBC/PLT):
Size vs. Intermediate angle light scatter
Size (ALL) is plotted on the x-axis and intermediate light scatter 3 on the y-axis. This facilitates separation of platelets from RBC and is useful when microcytes or RBC fragments are present. In this plot, IAS3 is measuring the internal complexity, which facilitates RBC and platelet separation.
FIGURE 10. IAS2 x IAS3 (RBC/PLT):
Intermediate angles of light scatter
The platelet population (yellow) and RBC (red) are separated using two intermediate angles of light scatter. Differences in both size and internal complexity of RBC vs platelets are evaluated.
FIGURE 11. IAS2 x ALL (RBC/PLT):
Intermediate angle light scatter vs. size
The platelet population (yellow) and RBC (red) are separated based on intermediate light scatter and size.
FIGURE 12. ALL x FL1 (RETIC/PLT)* :
Size vs. fluorescence
This scatterplot originates from the reticulocyte analysis where a nucleic dye separates reticulocytes (green) from mature RBC (red) based on staining of residual RNA found in reticulocytes. Platelets (yellow), and reticulated platelets (blue) which are FL1 positive, are shown to the left.

*Visible only when reticulocyte count has been ordered.
Alinity hq Casebook. Normal Case. ADD-00061876-v3 2020. p.12-15.

Alinity h-series is available in select countries, not including the US.
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The Alinity, ACCELERATOR and CELL-DYN systems are intended for performing in vitro diagnostic assays on samples of human origin (blood, urine). Read the instructions in the system manuals and labeling and/or reagent instructions carefully. Manufacturer or Authorized Representative: Abbott Germany.

Alinity h-series is available in select countries, not including the US.