Don't have an account? Sign Up

MAPSS™ Technology

The Multi-Angle Polarized Scatter Separation (MAPSS™) technology from Abbott uses four light scatter detectors to determine various cellular features. The application of a depolarized light detector is a unique characteristic of this method and allows for specific identification of eosinophil granulocytes.

The four detectors generate the following signals:

  • 0° or Axial Light Loss (ALL): related to size
  • 0° to 10° Intermediate Angle Scatter (IAS): related to cellular complexity
  • 90° Polarized Side Scatter (PSS): related to nuclear lobularity/segmentation
  • 90° Depolarized Side Scatter (DSS): related to eosinophil granules


These signals correlate with morphological characteristics that can be determined visually under the microscope from a stained slide. Various combinations of these four measurements are used to classify the WBC subpopulations and provide morphological flagging. Fluorescent flow cytometry is used to detect NRBCs based on DNA staining when additional reagent and a detector for fluorescence signal is added.1


Multi-dimensional cell classification using patented Multi Angle Polarized Scatter Separation (MAPSS™) plus 3-color Fluorescent technology provides analysis of both the White Blood Cell count and the differential. MAPSS™ plus 3-color Fluorescence uses fluorescent flowcytometric measurements of multiple angles of light scatter of up to 20,000 cellular events. This unique process enables multiple scatterplots to be produced to further the characterization of cellular events.1



MAPSS™ technology provides:

  • Identification and enumeration of WBCs and subpopulations
  • Identification of abnormal cell types
  • Identification of interfering substances thereby giving:
    – First pass identification of NRBCs.
    – Accurate Identification of Platelet Clump.1


CELL-DYN Sapphire

Access online product training, operator’s manual and other CELL-DYN Sapphire training & educational content.


Access online product training, operator’s manual and other CELL-DYN Ruby training & educational content.


Digital Resource
MAPSS™ Technology Video
Digital Resource
The Evolution of White Blood Cell Differential Technologies
On-Demand Webinar
Advancements in WBC Differential Technology
On-Demand Webinar
Principles and Performance Characteristics of Automated Platelet Counting Methods
1. Dr, Izzuna Mudla bt Mohamed Ghazali, Miss Sin Lian Thye, CELL-DYN Sapphire, Health Technology Assessment Unit Medical Development Division, Ministry of Health Malaysia. 021/07 , Decmber 2007.
©2022 Abbott, Abbott Park, Illinois, U.S.A.

Privacy Policy| Terms and Conditions

This website is designed to provide practical and useful information on the subject matter covered. However, it is provided [for free] with the understanding that Abbott is not in the business of providing scientific education. If medical, scientific, or other expert assistance is required, the services of a competent professional should be sought.

Unless otherwise specified, all product and service names appearing in this Internet site are trademarks owned by or licensed to Abbott, its subsidiaries or affiliates. No use of any Abbott trademark, trade name, or trade dress in this site may be made without the prior written authorization of Abbott, except to identify the product or services of the company.

All Alinity hq, CELL-DYN Ruby and CELL-DYN Sapphire instruments are Class I laser products. ACCELERATOR a3600 is a Class II laser product. 

The Alinity, ACCELERATOR and CELL-DYN systems are intended for performing in vitro diagnostic assays on samples of human origin (blood, urine). Read the instructions in the system manuals and labeling and/or reagent instructions carefully. Manufacturer or Authorized Representative: Abbott Germany.

Alinity h-series is available in select countries, not including the US.